Identification of 1,25-dihydroxycholecalciferol, a form of vitamin D3 metabolically active in the intestine.
نویسندگان
چکیده
A biologically active vitamin D(3) metabolite ("peak V metabolite") more polar than 25-hydroxycholecalciferol has been isolated from chicken intestines in pure form as a mono(trimethylsilyl)ether derivative. The molecule has been identified as 1,25-dihydroxycholecalciferol by means of mass spectrometry, ultraviolet absorption spectrophotometry, and specific chemical reactions.
منابع مشابه
The stimulation of 1,25-dihydroxycholecalciferol metabolism in vitamin D-deficient rats by 1,25-dihydroxycholecalciferol treatment.
Daily oral administration of 1,25-dihydroxycholecalciferol to vitamin D-deficient rats increases the rate of disappearance of [(3)H]1,25-dihydroxycholecalciferol and increases the rate of appearance of metabolites both less polar and more polar than 1,25-dihydroxycholecalciferol in the intestine, bone, liver, kidney, plasma, and muscle. Since 1,25-dihydroxycholecalciferol is believed to be the ...
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The prior administration of actinomycin D prevents the metabolism of [(3)H]25-hydroxycholecalciferol to 1,25-dihydroxycholecalciferol, a metabolite of vitamin D(3) that is effective in the stimulation of intestinal calcium transport. In this paper, the question of whether the response of intestinal calcium transport to 1,25-dihydroxycholecalciferol is sensitive to actinomycin D was examined. Wh...
متن کاملRegulation by calcium of in vivo synthesis of 1,25-dihydroxycholecalciferol and 21,25-dihydroxycholecalciferol.
Tritiated 1,25-dihydroxycholecalciferol accumulates in several tissues, to an extent that varies with dietary calcium content, 12 hr after the administration of 325 pmoles of tritiated 25-hydroxycholecalciferol to rats. As the dietary and serum calcium concentrations increase, the amount of 1,25-dihydroxycholecalciferol is diminished and the concentration of 21,25-dihydroxycholecalciferol incre...
متن کاملConcentration-Effect Relationship of Intrapritoneal Administration of 1, 25 (OH) 2-Vitamin D in a Chronic Constriction Model of Neuropathic Pain
Introduction: Results: These findings revealed the exaggerated responses in the group which received CCI. The group which was treated by 1 μg/kg of 1,25 vit D3 showed a significant reduction in pain behavior. Injection of 1,25 vit D3 did not change the response of animals to the acetone drop and von frey filament. Discussion: Our results showed that antinoceptive effect of 1,25 vit D3 in a rod...
متن کاملSynthesis of 1,25-dihydroxycholecalciferol and 24,25-dihydroxycholecalciferol by calvarial cells. Characterization of the enzyme systems.
The synthesis of 1,25-dihydroxycholecalciferol [1,25(OH)2D3] and 24,25-dihydroxycholecalciferol [24,25(OH)2D3] from 25-hydroxycholecalciferol [25(OH)D3] has previously been shown to occur in cells isolated from bone. The main findings of the present study are that the enzyme systems which catalyse these syntheses are: (1) active at 'in vitro' substrate concentrations over the range of 2-50 nM; ...
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ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 68 4 شماره
صفحات -
تاریخ انتشار 1971